1. To mark the boundary of an area with or as if with stakes. A noun or pronoun can be used between "stake" and "off." He staked the area of the field off that we could use for our crops. Please stake off the part of the lawn you'd like sprayed for weeds.

2. To claim or reserve something or some area for oneself. A noun or pronoun can be used between "stake" and "off." Why don't you go in ahead of us and stake off a few seats in the theater? Dad's going to stake a spot off for us on the field to watch the fireworks.