1. Literally, to step backward a single step. Everyone take a step back and give him a bit of space. They took a step back to get a better view of the painting.

2. To withdraw or remove oneself from something, often in order to consider it from a broader or more objective perspective. You're too focused on this project. You need to take a step back and consider the bigger picture of the whole company. I think everyone needs to take a step back from this issue for a moment and think about their motivations a bit more critically.